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Pci neo vector promega

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The pCI-neo Mammalian Expression Vector carries the human cytomegalovirus (CMV) immediate-early enhancer/promoter region to promote constitutive expression of cloned DNA inserts in mammalian cells. The pCI-neo Vector contains the neomycin phosphotransferase gene, a selectable marker for mammalian cells.

pAdVAntage™ Vector. When co-transfected in mammalian cells, this vector enhances transient protein expression by increasing translation initiation. E1711 pCI 4,006 Y T7 N Y Y T7 EEV pCI-neo 5,472 Y T7, T3 N Y Y T3, T7 EEV Key: S - SP6 M - M13 *Note: The T7 EEV (Eukaryotic Expression Vector) Promoter Primer (Cat.# Q6700) is designed specifically for use with Promega’s mammalian expression vectors. pCI-neo-ER vector was constructed by insertion of human ER cDNA derived from pIC-ER-F (ATCC, Manassas, Virginia, U.S.A.) in pCI-neo mammalian expression and neomycin resistant vector (Promega, Madison, Wisconsin, U.S.A.).

Pci neo vector promega

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Multiple cloning sites exist for easy insertion of cDNA. References Plasmid pCI-Neo-PTPRT (WT) from Dr. Bert Vogelstein's lab contains the insert PTPRT and is published in Science. 2004 May 21. 304(5674):1164-6. This plasmid is available through Addgene.

This vector also contains the neomycin phosphotransferase gene, a selectable marker for stable transfection of mammalian cells. The pCI-neo Vector can be used for transient or stable expression by selecting transfected cells with the antibiotic G-418. Multiple cloning sites exist for easy insertion of cDNA. References

Pci neo vector promega

pCMVTNT™ Vector ( #L5620) //promega.widen.net/content/wzc3rer15e/png/3603MA-W.png?w=600&h=400&position=c&color=ffffff&quality=80&u=7fvzhm. Plasmid pCI-neo Flag HAUSP from Dr. Bert Vogelstein's lab contains the insert HAUSP and is published in Cell Cycle.

Pci neo vector promega

The pCI-neo Mammalian Expression Vector carries the human cytomegalovirus (CMV) immediate-early enhancer/promoter region to promote strong, constitutive expression of cloned DNA inserts in mammalian cells. A β-globin/IgG chimeric intron located downstream from the enhancer/promoter region can further increase expression.

Pci neo vector promega

The pCI-neo Vector contains the neomycin phosphotransferase gene, a selectable marker for mammalian cells. Promega pCI and pCI-neo Mammalian Expression Vectors. Contains the CMV immediate-early The pCI-neo Mammalian Expression Vector carries the human cytomegalovirus (CMV) immediate-early enhancer/promoter region to promote strong, constitutive expression of cloned DNA inserts in mammalian cells.

The pCI-neo Vector can be used for transient or stable expression by selecting transfected cells with the antibiotic G-418. pCI-neo Mammalian Expression Vectorは、哺乳動物細胞における安定した遺伝子発現に最適な発現ベクターです。抗生物質G418を用いてトランスフェクト細胞を選択すれば、一過性の発現または安定な発現に利用できます。 Promega pCI and pCI-neo Mammalian Expression Vectors Contains the CMV immediate-early Promega Corporation is a worldwide leader in applying biochemistry and molecular biology to the development of innovative, high-value products for the life sciences. The Promega mission statement is: To be the most responsive supplier of biological reagents and reagent systems used in research and applied technology applications worldwide.

To see this sequence with restriction sites, features, and translations, please download SnapGene or the free SnapGene Viewer. Product Availability Update. Promega Manufacturing and Delivery Systems continue to be fully operational during the COVID-19 outbreak. Our teams are in regular contact with suppliers and distributors worldwide and are taking all steps necessary to address both demands for diagnostic tools and reliable delivery of all products as quickly as possible. The pCI Mammalian Expression Vector promotes constitutive expression of cloned DNA inserts in mammalian cells.

The expression of prME was under the control of the cytomegalovirus (CMV) promoter. For the pCI-D2prME-VSVG, pCI-D2prME-CD4 pCI-ZprMEVSVG, or pCI-ZprMECD4 vector, the transmembrane and cytoplasmic domain (TM) of the DENV2 or ZIKV E gene were replaced with the TM gene of the VSV G or the CD4 pCI-neo Mammalian Expression Vector. DNA cloned into this vector is constitutively expressed in mammalian cells; use the neomycin resistance gene for stable expression. E1841. pAdVAntage™ Vector. When co-transfected in mammalian cells, this vector enhances transient protein expression by increasing translation initiation.

Pci neo vector promega

The pCI-neo Mammalian Expression Vector carries the human cytomegalovirus (CMV) immediate-early enhancer/promoter region to promote strong, constitutive expression of cloned DNA inserts in mammalian cells. A β-globin/IgG chimeric intron located downstream from the enhancer/promoter region can further increase expression. pCI-neo Mammalian Expression Vectorは、哺乳動物細胞における安定した遺伝子発現に最適な発現ベクターです。抗生物質G418を用いてトランスフェクト細胞を選択すれば、一過性の発現または安定な発現に利用できます。 The pCI Expression Vector contains the human cytomegalovirus (CMV) major immediate-early gene enhancer/promoter region. A β-globin/IgG chimeric intron located downstream of the enhancer/promoter region can further increase expression in the pCI Vector.

pCMVT n T™ Vector contains a CMV enhancer/promoter region, which enables strong constitutive expression in many cell types, while RNA phage promoters enable RNA synthesis in vitro. pCI-neo Mammalian Expression Vector. DNA cloned into this vector is constitutively expressed in mammalian cells; use the neomycin resistance gene for stable expression. E1841.

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プロトコル. 英語 製品マニュアル. 英語 ベクターマップ＆シークエンス. パフォーマンス. 日本語 pCI-neo ベクターとpcDNA4/HisMax ベクターの比較;. 英語 

pAdVAntage™ Vector. When co-transfected in mammalian cells, this vector enhances transient protein expression by increasing translation initiation.

Briefly, a 895-bp fragment corresponding to nucleotides 162-1056 in the Elovl3 cDNA was cloned into a pCI-neo vector (Promega), appropriately linearized, and in vitro transcribed to obtain antisense and sense probes.

See all citations. Similar Products. pCI-neo Mammalian Expression  プロトコル. 英語 製品マニュアル. 英語 ベクターマップ＆シークエンス. パフォーマンス. 日本語 pCI-neo ベクターとpcDNA4/HisMax ベクターの比較;.

A β-globin/IgG chimeric intron located downstream from the enhancer/promoter region can further increase expression. pCI-neo. Mammalian cell expression vector with the CMV promoter and a neomycin (G418) resistance marker. To see this sequence with restriction sites, features, and translations, please download SnapGene or the free SnapGene Viewer. Product Availability Update.